Rovers Under-23s will take on Stoke City in the last 16 of the Premier League Cup.
The tie will take place at Leek Town’s Harrison Park on Friday March 15th, kick-off 7pm.
Damien Johnson's side booked their place in the knockout phase after defeating Doncaster Rovers 5-1 last week to finish second in Group B.
Rovers won three and drew one of their six group games to finish on 10 points, four points behind group winners Everton.
Stoke City topped Group A, winning four and drawing one of their six group games.
The two teams met twice in Premier League 2 Division 2 last season, with Rovers winning 2-0 at home and 1-0 away en route to lifting the title.