Jacob Davenport, who has had his season hampered by injury since signing from Manchester City in the summer, is expected to play a part in Southport this evening.
The 20-year-old is yet to play a competitive game for the first team and suffered a setback after initially making his comeback in a couple of Under-23s games earlier this season.
Davenport's return could provide a boost for Damien Johnson's side, who have had a number of departures in recent weeks, both on loan and permanently.
The release of Willem Tomlinson and Okera Simmonds last week followed on from Ollie Byrne, Joe Grayson, Sam Hart, Lewis Mansell and Brad Lyons all departing towards the end of the transfer window.
One who also won't be playing is regular goalkeeper Andy Fisher, who today made the switch to National League North outfit FC United of Manchester on a month-long loan deal.
New boy Louie Annesley could make his his first start for the side after the Gibraltar international centre back came off the bench in the 7-1 home demolition of Leicester City on Monday evening.
A win tonight will keep Rovers in second place, with just one game left in the Premier League Cup group stage following the full time whistle.
Defeat, however, could prove to be devastating for Johnson's hopes of progression, with third placed Sheffield United, who are level on points with Rovers in Group B, taking on Doncaster Rovers at 2pm this afternoon.
The hosts could include defender Brendan Galloway in the lineup, who has appeared 21 times in the Everton first team since signing from MK Dons in 2014.
Rovers will be looking for revenge against David Unsworth's side, who won at Leyland in the last Premier League Cup meeting back in November.
Kick-off at the Merseyrail Community Stadium is at 7pm.