Matty Platt has become the third young Rovers player to make a move into the non-league game in the space of 24 hours.
The towering defender has joined National League North side Southport on a month-long loan deal until early March.
The 21-year-old centre back follows in the footsteps of Andy Fisher and Lewis Hardcastle, who joined FC United of Manchester and Barrow AFC respectively on Friday, in search of first team football.
A Rovers Academy graduate, Platt has captained the club's Under-23s on numerous occasions and played a key role in their Premier League 2 Division 2 title success last season.
Platt previously had a loan spell with Barrow during the 2016-17 season and spent the first half of the current campaign on loan at Accrington Stanley, but was recalled by Rovers in January after making just five appearances for John Coleman's side, all in the cup competitions.
The young defender, who is expected to go straight into the Southport squad for today's game against Chorley, could come up against Rovers team-mate Fisher on February 23rd when the Port play FC United of Manchester at the Merseyrail Community Stadium.
Speaking about the trio's loan moves, Under-23s head coach Damien Johnson said: "I think they're all at that age where they need to be playing some first team football.
"Lewis has found his game time here a bit limited, so he was keen to explore other opportunities, and Matty has had a couple of frustrating loan spells, so he just wanted to go somewhere where he could get some games.
"We wish them all the best, we'll keep an eye on them and hopefully they can improve and get the experience they need."