Damien Johnson's Under-23s side are back in action today in the Lancashire Senior Cup against Accrington Stanley.
The winners of the tie, which will be played at Rovers' Academy base in Brockhall Village, will head through to the final of the competition.
Following a number of departures in the January window, and at the beginning of this month, boss Damien Johnson has something of a selection headache for today's clash.
One who won't be playing is regular goalkeeper Andy Fisher, who last made the switch to National League North outfit FC United of Manchester on a month-long loan deal.
Likewise, Matty Platt and Lewis Hardcastle both made moves out on loan last week, and won't be eligible to play for Rovers against Stanley.
New boy Louie Annesley could continue in the starting lineup after an impressive showing during the 1-1 draw against Everton Under-23s in the Premier League Cup clash on Friday night.
Rovers secured a bye in the first round, after Preston North End were unable to fulfil the fixture, before Damien Johnson’s side thrashed Blackpool 6-0 to book their place in the last four.
John Buckley, Jack Vale, Joe Grayson, Okera Simmonds and Jack Evans were all on target in the emphatic victory over the Seasiders.
Accrington won 4-0 away at Morecambe in the first round, before beating Bury 3-0 in the quarter-finals.
It is the third season in succession that Rovers have reached the semi-final stage, suffering penalty shoot-out defeats to Fleetwood and Bury in the last two years.
Supporters are welcome to attend the game, with entry free of charge. In the event of the scores being level at the end of 90 minutes, the winner of the tie will be determined by penalties.
The other semi-final sees Rochdale host Burnley.