Rovers have been drawn away to Premier League side Sheffield United in the second round of the Carabao Cup.
The tie will take place at Bramall Lane during the week commencing Monday August 26th.
Tony Mowbray's men booked their place in the draw courtesy of a dramatic 3-2 victory over Oldham Athletic at Ewood Park, whilst the Blades enter the competition at the second round stage.
It will be the first ever meeting between the two teams in the League Cup.
Sheffield United did the double over Rovers in the Championship last season, winning 2-0 at Ewood Park in October and 3-0 at Bramall Lane in December, on their way to securing automatic promotion to the Premier League.
Speaking after the draw was made, manager Tony Mowbray said: "I would have prefered a lower league team at home, but we've got Sheffield United away and let's hope they make a lot of change and we go there and put on a performance that we know we can find away from home.
"Sheffield United were the second best team in the Championship last season and they've got a deep squad now that they're in the Premier League.
"They've added some good players to it, so we know that it will be tough ask, but I don't think it will be one of those nights when the stadium will be bouncing and their team will be right up for it.
"Being a Premier League side now, they will probably make some changes and see how the game goes for them. For us, we have to get the balance right of experience and youth, and we'll look forward to the challenge.
"It's a cup competition and I think we're all going to be judged this year on how close we can get to the top six and how good a season we can have in the league rather than thinking about the cups and yet we'll try to progress.
"We'll go there, we won't disrespect it, because we know it's a tough ask and so we'll pick a team to try and get a result."
The second round draw in full:
Crewe Alexandra v Aston Villa
Lincoln City v Everton
Leeds United v Stoke City
Sheffield United v Rovers
Rotherham United v Sheffield Wednesday / Bury
Newcastle United v Leicester City
Burton Albion v Morecambe
Burnley v Sunderland
Nottingham Forest v Derby County
Grimsby Town v Macclesfield Town
Preston North End v Hull City
Rochdale v Carlisle United
Plymouth Argyle v Reading
Crawley Town v Norwich City
Newport County v West Ham United
Oxford United v Millwall
Watford v Coventry City
Swansea City v Cambridge United
Cardiff City v Luton Town
Bristol Rovers v Brighton & Hove Albion
Crystal Palace v Colchester United
Fulham v Southampton
AFC Bournemouth v Forest Green Rovers
Southend United v MK Dons
Queens Park Rangers v Portsmouth