Rovers manager Tony Mowbray believes key decisions went against his side during Tuesday's Checkatrade Trophy match against Rochdale.
The Blues went ahead through Joe Nuttall, despite having Scott Wharton sent off, but the match went to penalties following Jordan Slew's second-half equaliser.
Rovers eventually lost 5-3 in the shoot-out, meaning the outcome of the other game in Group C will determine our fate in the competition.
Speaking after the game, Mowbray said: "It was a good solid performance, I think we played for over an hour with 10 men and worked extremely hard.
"Having watched it back, it was a shocking decision for their equaliser, the boy was miles offside, but never mind.
"We tried to respect the competition but putting a strong team out, and to lose on penalties is a disappointment because it was a good performance under difficult circumstances.
"You have to ask the question 'was it a red card?' as well. I didn't see it because the linesman was in my way but, if it is, it's a young player making a rash challenge, probably.
"It's a strange decision so early in the game. If anything, Scott needs a bit more malice, playing in that position. He's hugely disappointed in that dressing room, but it's a learning curve for him.
"You want your defenders going for every ball, so it's the wrong decision really. It wasn't just that one, he wasn't quite sure about a few things and a lot went against us.
"We have to accept it and take the positives of a gritty performance with 10 men. It's not a case of losing points as we would in a league game."
Mowbray praised his youthful side for their performance, adding: "I thought we defended really well, we frustrated Rochdale and Nuttall was a threat on his own.
"It was okay. I was disappointed for them with not winning the shoot-out, but as a performance in terms of looking like we could score with 10 men against 11, it was okay on the night."