Rovers Ladies' remarkable 28-game unbeaten league run came to an end as they were beaten 2-0 by Huddersfield Town.
Gemma Donnelly's side had the better of the chances throughout, but Emily Heckler scored a goal in each half to inflict a first league defeat on Rovers since May 2016.
Rovers could - and perhaps should - have taken the lead after just a minute when Meghan Taylor’s shot was saved and Saffron Jordan fired the rebound straight at the keeper with the goal at her mercy.
Jordan went close again when Jess Holbrook's corner was headed back across goal, before being cleared off the line.
Taylor then had an effort cleared en route to goal, before the Terriers struck a sucker punch just before the break when Kate Mallin's cross from the right was headed home by Heckler.
Rovers started the second half on the offensive when centre back Kayleigh McDonald beat two players, before her low effort was well saved.
The hosts were handed a golden opportunity to get back into the game when Jordan was brought down in the box, which saw Huddersfield reduced to 10 players, but Lynda Shepherd saw her spot-kick saved by the impressive Laura Carter in the Huddersfield goal.
Despite dominating the ball and creating countless chances, Rovers were made to pay 15 minutes from time when Heckler volleyed home her second of the game from the edge of the area.
After the game, manager Donnelly said: "It had to come to an end at some stage.
"Well done to Huddersfield - they played a great game and deserved the result.
"For us, we raise the bar and we go again."
ROVERS: Hill, Jukes, Cook, McDonald, Pearson, Shepherd (McCoy 73), Holbrook, Fenton, Jordan, M. Taylor (Makin 46), Cunliffe (A. Taylor 46). Subs not used: Montgomery.