Rovers' development squad have been drawn with Southampton, Watford and Cardiff City in this season's Premier League Cup.
Following the pre-qualifying stage, Damien Johnson's side enter at the group stage and face home and away matches against the three sides.
Rovers met Cardiff City in last season's competition, claiming four points off the Bluebirds. Whilst in its previous guise, Rovers were defeated by Southampton in the 2015 final.
The top two teams from each of the eight groups go through to the last 16 of the competition, which are one-off knockout ties from then on.
Rovers progressed through the group stages last season before being knocked out by eventual winners Swansea City.
Dates for the six Premier League Cup matches will be confirmed in due course.