Tony Mowbray felt Rovers were on the wrong side of a contentious refereeing decision in the 1-1 draw with Bristol City.
The visitors went in front early thanks to Tammy Abraham's 25th goal of the season, and it wasn't until Rovers' drew level in the 71st minute thanks to Sam Gallagher's header that the home side posed any meaningful threat.
However the goal brought about a change of momentum with Gallagher seeing huge claims for a penalty waved away when Robins' captain Korey Smith brought the striker down from behind. A key decision that the Rovers boss believed the officials had got wrong.
"We've all looked at it in the dressing room and I believe it should have been a penalty," Mowbray told Rovers PlayerHD.
"He's inside his man, he's running through on goal, he's in front of him and the boy actually falls on the back of his legs as he's going through to score. It'll be interesting to know why he didn't give the penalty, I'm not sure what his reasoning not to give it would be.
"So it is a real frustration of course because we were in a spell where the crowd were right up for the game again and were right behind the team. The team had some belief and it would have been a fantastic result to get a penalty to score to win 2-1.
"We played against a decent side who you could see were good with the football, we tried to find a win in the second half but we didn't quite get there."
Mowbray felt Rovers struggled from the off against Bristol City, believing there was a nervousness to their play.
He added: "It was similar to Barnsley to be honest, we didn't play particularly well, there was a bit of nervousness about the team.
"They're treading on egg shells, we need to start with intensity, go for the throat from the off, play forward and get the crowd with you from the off.
"But that's the last couple of games where they haven't done that, so that is a disappointment. We have to play without fear or concern really at home and we've one more opportunity this season against Villa.
"So lets hope we can pick up three points in that and remain unbeaten until the end of the season and hope there is another three points as well somewhere along the line and lets see how we go."
Rovers are back in action this upcoming Saturday when they take on Wolverhampton Wanderers.
Tickets are on open sale for the match at Molineux priced at £30 Adults, £15 Seniors 65+ and Under 21’s £15, Under 17’s £12 and Under 12’s £10.
Buy online today at www.eticketing.co.uk/onerovers