Rovers' medical staff will assess Derrick Williams this Monday to discover the extent of his injury.
The defender limped out of Saturday's match against former club Bristol City just four minutes into the second half at Ashton Gate with a suspected hamstring strain.
Williams picked up the injury after stretching to block a cross, and although he attempted to play on, he was eventually replaced by Charlie Mulgrew.
"Derrick will need to be assessed, but it certainly wasn't great and that is why he had to come out of the game," said boss Owen Coyle.
"It is his hamstring so we'll have to assess the severity of the injury.
"Hopefully it won't be too long but the medical team will look at him and take if from there.
"Anthony Stokes had food poisoning at the start of the week and although he was able to train on Tuesday from there got steadily worse.
"There is no midweek game so hopefully we'll get one or two back from the treatment table to bolster the squad."
Rovers are back in action at Ewood Park next Saturday when Wolverhampton Wanderers visit in the 5.30pm kick-off.
Tickets are on open sale priced from £22 for Adults, £14 Concessions and £9 for Juniors aged 17 and under.
Buy online today at www.eticketing.co.uk/onerovers, in store at Ewood Park, or by calling 01254 372000.