Rovers have been handed a tough away draw in the third round of the FA Youth Cup.
Billy Barr's young side will face Premier League giants Arsenal in their opening fixture of the prestigious youth competition.
Rovers reached the semi-final stage last season, beating Southend United (1-0), Leicester City (3-2), Aston Villa (2-1) and Luton Town (1-0), before losing 4-1 on aggregate to eventual winners Chelsea.
The Gunners also made it through to the last four in 2015-16, before being knocked out by Manchester City over two legs.
A date and venue for the fixture will be confirmed in due course.
Third round draw in full:
Middlesbrough v Rotherham United
Liverpool v Crystal Palace
Boreham Wood/Broxbourne Borough v Chester/Bolton Wanderers
Burnley v Bradford City/Boston United
Watford v Norwich City
Plymouth Argyle/Gillingham v Oxford City/Peterborough United
Huddersfield Town v AFC Wimbledon/Bristol Rovers
Reading v Manchester City
Swindon Town/AFC Sudbury v Queens Park Rangers
Rochdale/Shrewsbury Town v Morecambe/Accrington Stanley
Portsmouth/Met Police v Newcaste United
Leicester City v Poole Town/Leyton Orient
Brighton & Hove Albion v Derby County
Exeter City/MK Dons v Wigan Athletic
Arsenal v Rovers
Preston North End v Halifax Town/Sheffield United
Romulus/Oldham Athletic v Birmingham City
Swansea City v Wolverhampton Wanderers
Sunderland v Burton Albion
Fulham v Mansfield Town/Dunkirk
Cambridge United/Millwall v Bournemouth
Barnsley v Chesterfield/Carlisle United
Chelsea v Cardiff City
Everton v Scunthorpe United/Tranmere Rovers
Stoke City v West Ham United
Nottingham Forest v Ipswich Town
West Bromwich Albion v Yeovil Town/Merstham
Hull City v Leeds United
Tottenham Hotspur v Stevenage/Charlton Athletic
Bristol City v Aston Villa
Sheffield Wednesday v Luton Town/Dagenham & Redbridge
Manchester United v Southampton