A goal in each half condemned Rovers' youngsters to defeat at the hands of Manchester City on Saturday.
Despite the impressive display last Wednesday against West Ham United, Billy Barr's side failed to repeat that against a City team that have all but secured top spot in the end of season play-off group.
Rovers struggled from the off and it came as no surprise when Jadon Sancho opened the scoring for the hosts after just 18 minutes.
Cutting in from the left the City forward got the better of the returning Matty Makinson before firing home.
Whilst Mason Fawns, Joel Steer and Stefan Mols only had half-chances to level, Rovers had keeper Ben Ascroft to thank for keeping City at bay with a string of fine saves throughout.
But the 17-year-old stopper was finally beaten three minutes into stoppage time at the end of the match when Sancho added his and City's second to complete the victory.
"It just goes to show the inconsistencies of Under-18s football," said Barr.
"We were poor, and it is all the more disappointing considering how well we played on Wednesday.
"But we couldn't match it on Saturday, our quality was lacking, we didn't create many chances, when we had possession we gave it away cheaply.
"We finish the season at home to Chelsea next week. We started this play-off group with a win and hope to end it with one as well."
ROVERS: Ascroft, Makinson, Askew (sub Williams 69 mins), Grayson, C. Doyle, Magloire, Fawns, Mols, Steer, Curran (sub Lynch 69 mins), Howarth.
Subs not used: Campbell, Butterworth.