The midfield duo both played for the development squad seven days ago, and will get more minutes under their belts at Leigh Sports Village.
Damien Johnson's side go into tonight's match looking to avoid a third straight defeat following losses against Fulham and Swansea City this past week.
However, they'll be looking to do the double over their opponents following a 3-2 win back in October.
Wolves raced into a two-goal lead inside the opening 15 minutes at Molineux, but a brace from Lewis Mansell and a debut goal from defender Matty Platt saw Rovers triumph on their travels.
Along with Guthrie and Lowe, this evening's squad includes fellow senior Simon Eastwood in goal, whilst Ryan Nyambe, Josh Askew, Scott Wharton and Lewis Hardcastle, who all impressed for the Under-18s in their FA Youth Cup semi-final with Chelsea last Friday all start.
ROVERS: Eastwood, Nyambe, Askew, Guthrie, Lavelle (c), Wharton, Mahoney, Lowe, Forrester, Hardcastle, Wall.
Subs: Platt, Fisher, Tomlinson, Rankin-Costello, Mansell.
WOLVES: McCarey, Odoffin, Deslandes, Rainey, Hayden, Leak, Armstrong, Herc, Reid, Enobakhare, Hunte.
Subs: Breslin, Simpson, Upton, Randall, Collins.
Kick-off at Leigh Sports Village is at 7.00pm, Rovers supporters can gain free admission this evening by completing this online form.