Rovers have been drawn away to League Two Mansfield Town in the first round of the EFL Cup.
The tie against Adam Murray's men will be played week commencing Monday August 8th.
Owen Coyle's side were due to face Mansfield in a friendly on Tuesday July 26th, but the planned pre-season fixture has now been called off.
The team will instead visit the One Call Stadium for the cup clash a fortnight later.
Rovers will be looking to make it into the second round of the competition for the first time in four years, having made early exits at the hands of Shrewsbury Town (2-1), Scunthorpe United (1-0) and Carlisle United (on penalties) in the past three seasons.
First round draw in full:
Carlisle United vs Port Vale
Rotherham United vs Morecambe
Accrington Stanley vs Bradford City
Rochdale vs Chesterfield
Mansfield Town vs Rovers
Barnsley vs Northampton Town
Cambridge United vs Sheffield Wednesday
Derby County vs Grimsby Town
Oldham Athletic vs Wigan Athletic
Scunthorpe United vs Notts County
Doncaster Rovers vs Nottingham Forest
Burton Albion vs Bury
Fleetwood Town vs Leeds United
Sheffield United vs Crewe Alexandra
Shrewsbury Town vs Huddersfield Town
Preston North End vs Hartlepool United
Blackpool vs Bolton Wanderers
Peterborough United vs AFC Wimbledon
Bristol Rovers vs Cardiff City
Coventry City vs Portsmouth
Luton Town vs Aston Villa
Brighton & Hove Albion vs Colchester United
Cheltenham Town vs Charlton Athletic
Birmingham City vs Oxford United
Southend United vs Gillingham
Wolverhampton Wanderers vs Crawley Town
Leyton Orient vs Fulham
Ipswich Town vs Stevenage
Wycombe Wanderers vs Bristol City
Walsall vs Yeovil Town
Exeter City vs Brentford
Queens Park Rangers vs Swindon Town
Barnet vs Millwall
Reading vs Plymouth Argyle
Newport County vs MK Dons