Gary Bowyer pointed to the contentious early sending off of Adam Henley as the pivotal moment as Rovers went down 3-0 away at MK Dons.
The Rovers defender was red-carded inside ten minutes for a soft challenge on Josh Murphy at stadiummk, a decision that was compounded as the home side converted the resulting penalty kick.
That left Rovers a man light for 80 minutes, and although they battled manfully, late goals eventually condemned them to defeat.
"In any game when you have a player sent off, and so early on as well, it has a massive bearing on it," said Bowyer. "But what you ask for is hopefully the decision is correct.
"The referee sprinted to give it and that is disappointing. Was the contact made in the penalty box? Was it a foul in the first place? These are all questions that the referee has to be very clear in his head, he has to be 100% sure that it is a foul.
"The use of technology helps him out massively but we continue to ignore it."
The penalty decision against Rovers wasn't the only one disputed on a disappointing afternoon in Buckinghamshire as Jordan Rhodes and Craig Conway both saw strong claims waved away, but the boss was delighted with the spirit and endeavour shown by the ten men.
"You've seen the performance of them, in the second half they were terrific," he added.
"The response from them, you'd have never been able to tell we were down to ten men.
"The goalie has made a hell of a save from Jordan, we've had one cleared off the line from a Shane Duffy header. We had the belief and confidence that we would get back into the game.
"But we've not defended the second well enough, although it was a fantastic cross and that left us with too much to do.
"It is important now that we regroup, look after everyone and bounce back on Wednesday night."
Rovers are back in action this Wednesday night when Derby County are the visitors to Ewood Park.
Tickets are currently on open sale priced from £18 for Adults, £12 for Seniors 65 and over, £12 for 18-21 year-olds and just £7 for Juniors aged 17 and below.
Book online today at www.eticketing.co.uk/onerovers and collect your tickets on the evening of the match at the Roverstore collection point.