Greenbank High School will represent Rovers in the Football League Girls Cup after winning the North West finals at the club's Indoor Centre.
The cup competition comprises of a series of six-a-side tournaments for under-13 girls, which culminates in a grand final prior to the Johnstone's Paint Trophy final at Wembley Stadium in April.
A total of 19 teams and around 150 girls took part in the North West event, with the Southport school coming out on top.
Andrew Aspinall, Girls' Development Officer at Blackburn Rovers Community Trust, said: "The tournament is a brilliant opportunity for the club to encourage more girls to start playing football with their friends and to give more playing opportunities to those who already play girls football.
"We stressed the importance for schools to work closely with their local grassroots teams in order to create a pathway for girls who want the opportunity to play more football to be able to play and train each week with their local clubs.
"It is really important that Blackburn Rovers work closely with local clubs, managers, teachers and coaches in order to grow girls football in Lancashire."