Rovers have made just the one change from the side that drew 0-0 with the Saints seven days ago.
Devarn Green misses out with the hamstring strain picked up in the first leg, Lewis Mansell, his replacement at Ewood, comes in to start.
The hosts have named the same 16-man squad from the previous match.
SOUTHAMPTON: Gazzaniga, Mugabi, McCarthy (c), Wood, Targett, Gape, Reed, Hesketh, Gallagher, Seager, McQueen.
Subs: Britt (GK), Sims, Sinclair, Slattery, Debayo.
ROVERS: Raya, Nyambe, Doyle, Thomson, Lavelle, Jones, Rittenberg, Bauress, Mansell, Carson (c), Mahoney.
Subs: Crump (GK), Daly, Tanner, Hardcastle, Joel.