Boss Gary Bowyer was left to lament being on the wrong end of crucial decisions as they lost 3-1 to Norwich City on Tuesday night.
Rovers went in front thanks to Tom Cairney's 25-yard thunderbolt after just 41 seconds and were then denied a stonewall penalty after a clear handball in the box.
But after a strong Rovers start Norwich were handed a way back into the game when Jason Lowe was adjudged to have handled Javier Garrido's centre. Lewis Grabban following up after his initial penalty had been saved by Paul Robinson.
Rovers had to fight a rearguard action as the home side laid siege with their resolve eventually broken three minutes from time when Bradley Johnson curled a sumptuous effort beyond Paul Robinson.
Even after that Rovers had chances to equalise as Markus Olsson saw a header cleared off the line before Norwich raced away to add a third in stoppage time.
"We feel we've been on the wrong side of two major decisions in the game," said Bowyer. "It is as simple as that.
"We come out, we score an outstanding goal and we were very dominant for the first 15 minutes.
"We should have had a penalty, the lad's hand is in an un-natural position and yet it doesn't get given.
"Norwich come into the game, which they are going to do at home, they are a strong outfit, one of the favourites for promotion. But then to let them back in the game with the penalty that he gives, Lowey's hand is down by his side, I can't put it in words to explain it.
"Kyle Naughton gets sent off for Tottenham at the weekend and it is no different to that we've just seen that we don't get given. It is a massive disappointment."
Bowyer was delighted with the resolve shown under increasing pressure as the players fought to the last.
"The way we started, the way we've stayed in the game, even at the end when they go 2-1 up we still had two great chances," added the Rovers boss. "We kept going, we showed some fight.
"Our supporters were magnificent. One of the things that they'll be aware of as they come away from here is that we didn't lie down and die here tonight, we had a right good go.
"You saw at the end their emotion from beating us, they knew that it was a very very tough game for them."
Rovers are back in action this Saturday when AFC Bournemouth are the visitors to Ewood Park. Prices are from £12 Adults, from £9 Seniors/Students and from £5 for Under-17s. Buy online today at www.onerovers.co.uk and collect from the Blackburn End collection point on the day of the game.