Rovers' Under-18s paid the price for a slow start and a slack finish as they were defeated 5-1 by Bolton Wanderers at the weekend.
David Lowe's side were the victors when the two sides met this pre-season, but the Trotters got their revenge in the league encounter on Saturday.
The young Rovers gave themselves a mountain to climb by conceding two Jamie Thomas goals inside the first twenty minutes as poor defensive play proved costly.
The deficit was reduced before the break however as Stuart Callaway scored a fine goal. A long diagonal ball from Cameron Scott was flicked on by Sam Joel and Callaway controlled before firing into the back of the net.
Rovers started the second half strongly, creating several chances, but without reward. Adrian Edo fired over when well placed, Willem Tomlinson spurned a great chance, then substitute Ollie Mehew headed over.
With the Rovers pushing for an equaliser, they were hit three times before the close, Thomas scoring two further goals either side of a Carl Kennedy chip.
"The result is a huge disappointment because some of our football was good at times but we didn't make it count," said Lowe.
"We were in the ascendency for 20 minutes in the second half, dominating possession and creating chances to equalise.
"But we tired badly towards the end, lost our discipline, and defended sloppily."
The Under-18s are back on the road this weekend when they face Derby County.
ROVERS: Williams, Nyambe, Langford, Pierce, Scott, Platt, Howarth, Edo (sub Mehew 60 mins), Joel, Tomlinson, Callaway (sub Mansell 77 mins).
Subs not used: Pirretas, Fisher, Wharton.