Rovers' 14-match unbeaten league run, stretching back to March 2014, came to an end on Tuesday night when they were beaten 3-1 by Norwich City.
The Rovers boss saw the reaction to the defeat from his players and believes they'll want to take out that anger and frustration on Saturday against AFC Bournemouth.
"If ever there was a time for the supporters to see how committed our lads are, or how far we have come in a year, then if there was a camera in that dressing room they would have seen the hurt that was on the player's faces," said Bowyer.
"It is a long time since they've had that feeling of a defeat and I think the manner of which it came was the main source of anger and frustration. To have lost the record like that, it was hard for them.
"From a manager's point of view, the pride in terms of how far they have come in terms of their mentality was massive because it shows that we are making progress all the time and where we are wanting to get to with the attitude and commitment that these players have, we are going in the right direction.
"You look at the performances last season when they were on the run, what we will see now is how they'll respond. We challenged them straight away after the game.
"Now we go again and we've got to try and put another run together. I've no doubt that with the players that we've got now they'll go out and start again."
Tickets for Saturday's match against AFC Bournemouth are from £12 Adults, from £9 Seniors/Students and from £5 for Under-17s. Buy online today at www.onerovers.co.uk
and collect from the Blackburn End collection point on the day of the game.