When the two sides met at Leigh Sports Village back in February Rovers ran out 2-1 winners, and on this occasion they went one better at the Eppleton Colliery Welfare Ground.
Colin Hendry's side were quick off the mark, taking the lead after just three minutes. Micah Evans attacked the area and as his ball in was cleared out to the edge of the box John O'Sullivan cooly slotted into the bottom corner with a sidefooted effort.
Both sides worked hard but it was Rovers who eventually got the better of the game between two young sides.
A second goal duly arrived on 69 minutes through Jordan Slew, the striker latching onto a pass from Jamie Maclaren to shoot goalwards, the ball squirming under Black Cats 'keeper Jordan Pickford and into the back of the net.
The match also saw two debutants at Under-21 level as Academy players Modou Cham and Ian Vicars made second half appearances on a pleasing evening for Rovers.
Speaking after the match Colin Hendry praised his side for the way they went about the game: "We had to deal with quite a direct game of football tonight, and at times they gave our centre halves a difficult night. But once they got to grips with it I felt we coped with it well.
"We had square pegs in round holes at the back a little with two players out of position, but they did really well.
"We played some good stuff, tried to get the ball down and get the wide men in, we had some good attempts at goal.
"It was good to see Anton Forrester back after a few weeks out, whilst young Mo played 45 minutes and acquitted himself really well.
"It was a really good team performance."
The Under-21s are back in action next Monday evening when they face Middlesbrough at Ewood Park, kick-off 7.00pm.
SUNDERLAND: Pickford, Marrs, Ferguson, Laidler, Harrison, McNamee, Mitchell, Gorrin, Noble, Mandron, T. Robson.
Subs: E. Robson, Dixon, Honeyman, Agnew, Beadling.
ROVERS: Urwin, Fernandez, Bauress, Brown, Payne, Daly (c), Evans (sub Cham 46 mins), O'Sullivan, Forrester (sub Vicars 80 mins), Maclaren, Slew.
Subs: Dilo, Wassi.