Following the departure of Michael Appleton, Bowyer will take charge of the first team once more and insists that the fans have a huge part to play between now and the end of the season.
"The main thing for the supporters is to back the team the way they did against Burnley," said Bowyer.
"They backed the side right until the death and with Dunny scoring in injury-time was great for us and gave us the boost to go into this game.
"With the experience from sampling that atmosphere we want to appeal to the fans to come and cheer the football club and the players on once again.
"Because when the fans are like that the players respond and vice-versa, it is much needed."
The caretaker boss knows that a win has to be a must if they are to put some daylight between themselves and the sides at the bottom of the table.
He added: "We've got to try and win the game and get the team climbing up the league. You've always to be realistic and we have to try and get some wins as quickly as possible.
"Hopefully we can the start climbing the table. It has been a tough season and we want to finish it on a high now.
"We've got to treat it game by game, three more wins should see us right that is for sure."
Tickets for the Good Friday match will be on sale until the 3.00pm kick-off.
Prices start from £19 Adults, £12 Seniors and £7 Juniors. Book online today at www.onerovers.co.uk
and collect your tickets from the Blackburn End collection point on the day of the game.