Fabio Nunes earned his first cap at Under-21 level for Portugal on Monday evening.
The Rovers wide-man played the full ninety minutes for Rui Jorge's side against the Ukraine, but they lost the game 1-0 in Moreira de Conegos.
The only goal came in the 24th minute from Ukraine's Boris Taschy.
The friendly match was arranged after Portugal failed to qualify for next summer's UEFA Under-21 finals.