Jordan Slew lasted just 22 minutes at Fratton Park on Saturday as Oldham Athletic defeated Portsmouth 1-0.
The on-loan striker picked up a reoccurrence of a back injury and has now returned to Brockhall for treatment.
Fresh from his deadline-day loan move to Crystal Palace, David Goodwillie started on the bench as Crystal Palace picked up their first win of the new season with a 2-1 home victory over Sheffield Wednesday.
The Scottish striker played the last 27 minutes as the Eagles picked up maximum points at Selhurst Park.
Ryan Edwards was on the winning side for the first time this campaign as Rochdale defeated Barnet 2-0 at Spotland.
The central defender played the full match as goals from Ashley Grimes and Dele Adebola gave John Coleman's side all three points.
Anthony O'Connor again played the full match for Burton Albion, but they couldn't build on their shock midweek Capital One Cup win over Leicester City as they lost 3-0 away at Exeter City.
The only player not to feature on Saturday was Myles Anderson. He was an unused substitute as Aldershot Town lost 4-1 away at Fleetwood Town.
However, Myles was Rovers’ only representative in the Johnstone’s Paint Trophy on Tuesday night.
The versatile defender played a pivotal role as Aldershot beat League Two high-flyers Exeter 4-3 on penalties after the Southern Section first round tie had finished goalless.
Myles was making his first start for the Shots since his loan switch to the Recreation Ground and produced an impressive defensive display.